Incorporation of the enantiomers of lipoic acid into the pyruvate dehydrogenase complex from Escherichia coli in vivo

Biol Chem Hoppe Seyler. 1992 Jun;373(6):333-5. doi: 10.1515/bchm3.1992.373.1.333.

Abstract

The uptake of 35S-labelled enantiomers of lipoic acid into cells from Escherichia coli was studied. The R-enantiomer was taken up by a factor of two more efficiently than the S-form. Autoradiography of polyacrylamide gels of partially purified pyruvate dehydrogenase complex from these cells showed that only the R-lipoic acid was covalently incorporated as a cofactor into the dihydrolipoamide acetyltransferase component of the pyruvate dehydrogenase complex.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoradiography
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / enzymology*
  • Pyruvate Dehydrogenase Complex / chemistry
  • Pyruvate Dehydrogenase Complex / metabolism*
  • Spectrophotometry, Ultraviolet
  • Stereoisomerism
  • Thioctic Acid / analysis
  • Thioctic Acid / metabolism*

Substances

  • Pyruvate Dehydrogenase Complex
  • Thioctic Acid