Characterization of in vitro metabolites of rutaecarpine in rat liver microsomes using liquid chromatography/tandem mass spectrometry

Rapid Commun Mass Spectrom. 2004;18(10):1073-80. doi: 10.1002/rcm.1448.


Following incubation of rutaecarpine, a new cyclooxygenase-2 inhibitor, with rat liver microsomes, the structures of the metabolites were characterized by liquid chromatography with tandem mass spectrometry. Nine metabolites corresponding to mono- or dihydroxylated rutaecarpine were formed. Characteristic product ions for the identification of rutaecarpine metabolites were observed at m/z 136, 158 and 286. The loss of water led to the fragment ion at m/z 286, indicating the hydroxylation of the aliphatic ring. The fragment ion at m/z 136 indicated the hydroxylated form of the phenyl group of the quinazolinone moiety, while that at m/z 158 indicated the hydroxylated form of the aromatic ring of the indole moiety.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkaloids / analysis
  • Alkaloids / chemistry
  • Alkaloids / metabolism*
  • Animals
  • Chromatography, High Pressure Liquid / methods
  • Hydroxylation
  • In Vitro Techniques
  • Indole Alkaloids
  • Male
  • Microsomes, Liver / metabolism*
  • Molecular Structure
  • Protons
  • Quinazolines
  • Rats
  • Rats, Sprague-Dawley
  • Spectrometry, Mass, Electrospray Ionization / methods*


  • Alkaloids
  • Indole Alkaloids
  • Protons
  • Quinazolines
  • rutecarpine