Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 22 (3), 275-82

Controlled, Scalable Embryonic Stem Cell Differentiation Culture

Affiliations

Controlled, Scalable Embryonic Stem Cell Differentiation Culture

Stephen M Dang et al. Stem Cells.

Abstract

Embryonic stem (ES) cells are of significant interest as a renewable source of therapeutically useful cells. ES cell aggregation is important for both human and mouse embryoid body (EB) formation and the subsequent generation of ES cell derivatives. Aggregation between EBs (agglomeration), however, inhibits cell growth and differentiation in stirred or high-cell-density static cultures. We demonstrate that the agglomeration of two EBs is initiated by E-cadherin-mediated cell attachment and followed by active cell migration. We report the development of a technology capable of controlling cell-cell interactions in scalable culture by the mass encapsulation of ES cells in size-specified agarose capsules. When placed in stirred-suspension bioreactors, encapsulated ES cells can be used to produce scalable quantities of hematopoietic progenitor cells in a controlled environment.

Similar articles

See all similar articles

Cited by 71 articles

See all "Cited by" articles

Publication types

LinkOut - more resources

Feedback