Cleavage of type VIII collagen by human neutrophil elastase

Biochim Biophys Acta. 1992 Aug 25;1139(4):295-9. doi: 10.1016/0925-4439(92)90103-t.


In this report, the susceptibility of type VIII collagen to human neutrophil elastase is compared to other extracellular matrix components. Type X collagen is degraded to specific fragments at a substrate to enzyme ratio of 5:1 after 20 h at room temperature, but type VIII collagen is almost completely degraded after only 4 h incubation at a substrate to enzyme ratio of 50:1 and partly degraded after only 15 min. Laminin, merosin and types I, III, IV and V collagen exhibit no susceptibility to neutrophil elastase under the latter conditions, while fibronectin is degraded.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Collagen / chemistry
  • Collagen / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Fibronectins / metabolism
  • Humans
  • Laminin / metabolism
  • Leukocyte Elastase
  • Membrane Proteins / metabolism
  • Pancreatic Elastase / metabolism*
  • Substrate Specificity
  • Temperature


  • Fibronectins
  • Laminin
  • Membrane Proteins
  • Collagen
  • Pancreatic Elastase
  • Leukocyte Elastase