Growth of Piscirickettsia salmonis to high titers in insect tissue culture cells

T Harry Birkbeck; Angela A Griffen; Helen I Reid; L Anthony Laidler; Simon Wadsworth

doi:10.1128/IAI.72.6.3693-3694.2004

Growth of Piscirickettsia salmonis to high titers in insect tissue culture cells

Infect Immun. 2004 Jun;72(6):3693-4. doi: 10.1128/IAI.72.6.3693-3694.2004.

Authors

T Harry Birkbeck¹, Angela A Griffen, Helen I Reid, L Anthony Laidler, Simon Wadsworth

Affiliation

¹ Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, Scotland, United Kingdom. T.H.Birkbeck@bio.gla.ac.uk

Abstract

Piscirickettsia salmonis was grown in established insect, frog, and fish tissue culture cells. The yield of P. salmonis in Sf21 cells was up to 100 times that obtained in CHSE-214 cells, and virulence for Atlantic salmon was retained. The ceiling temperature for growth of P. salmonis in Sf21 cells was 24 degrees C.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteriological Techniques
Cells, Cultured
Fish Diseases / microbiology
Fish Diseases / mortality
Piscirickettsiaceae / growth & development*
Piscirickettsiaceae / pathogenicity
Piscirickettsiaceae Infections / microbiology
Piscirickettsiaceae Infections / veterinary
Salmo salar / microbiology*
Spodoptera / microbiology*
Virulence