Products in the T-state of aspartate transcarbamylase: crystal structure of the phosphate and N-carbamyl-L-aspartate ligated enzyme

Biochemistry. 2004 Jun 1;43(21):6422-6. doi: 10.1021/bi0302144.

Abstract

The structure of aspartate transcarbamylase of Escherichia coli ligated to products (phosphate and N-carbamyl-l-aspartate) has been determined at 2.37 A resolution (R-factor = 0.23, R(free) = 0.27). Results might indicate a product release mode, rather than close analogues to the transition state like those found in our earlier studies of other ligands (N-phosphonacetyl-L-aspartate, carbamyl phosphate plus malonate, phosphonoacetamide plus malonate, or citrate plus phosphate). Ordered product release, first carbamylaspartate (CLA) and then phosphate, might be facilitated by a 4 A movement of phosphate from the substrate-analogue position to the product (phosphate) binding position, and by a somewhat similar release movement of the other product (CLA) relative to its analogue (citrate). This movement is consistent with earlier studies of binding of either pyrophosphate or phosphate alone [Honzatko, R. B., and Lipscomb, W. N. (1982) J. Mol. Biol. 160, 265-286].

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aspartate Carbamoyltransferase / chemistry*
  • Aspartate Carbamoyltransferase / metabolism*
  • Binding Sites
  • Carbamyl Phosphate / chemistry
  • Carbamyl Phosphate / metabolism*
  • Crystallography, X-Ray
  • Models, Molecular
  • Phosphates / chemistry
  • Phosphates / metabolism*
  • Protein Conformation

Substances

  • Phosphates
  • Carbamyl Phosphate
  • Aspartate Carbamoyltransferase

Associated data

  • PDB/1R0C