New synaptic bouton formation is disrupted by misregulation of microtubule stability in aPKC mutants

Neuron. 2004 May 27;42(4):567-80. doi: 10.1016/s0896-6273(04)00255-7.

Abstract

The Baz/Par-3-Par-6-aPKC complex is an evolutionarily conserved cassette critical for the development of polarity in epithelial cells, neuroblasts, and oocytes. aPKC is also implicated in long-term synaptic plasticity in mammals and the persistence of memory in flies, suggesting a synaptic function for this cassette. Here we show that at Drosophila glutamatergic synapses, aPKC controls the formation and structure of synapses by regulating microtubule (MT) dynamics. At the presynapse, aPKC regulates the stability of MTs by promoting the association of the MAP1Brelated protein Futsch to MTs. At the postsynapse, aPKC regulates the synaptic cytoskeleton by controlling the extent of Actin-rich and MT-rich areas. In addition, we show that Baz and Par-6 are also expressed at synapses and that their synaptic localization depends on aPKC activity. Our findings establish a novel role for this complex during synapse development and provide a cellular context for understanding the role of aPKC in synaptic plasticity and memory.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actin Cytoskeleton / metabolism
  • Animals
  • Caenorhabditis elegans Proteins / metabolism
  • Carrier Proteins / metabolism
  • Cell Differentiation / genetics*
  • Drosophila Proteins / metabolism
  • Drosophila melanogaster / genetics
  • Drosophila melanogaster / growth & development*
  • Drosophila melanogaster / metabolism
  • Glutamic Acid / metabolism
  • Intracellular Signaling Peptides and Proteins*
  • Memory / physiology
  • Microtubule-Associated Proteins / metabolism
  • Microtubules / metabolism*
  • Mutation / genetics
  • Nerve Growth Factors / metabolism
  • Nervous System / cytology
  • Nervous System / growth & development*
  • Nervous System / metabolism
  • Neuronal Plasticity / genetics
  • Presynaptic Terminals / metabolism*
  • Presynaptic Terminals / ultrastructure
  • Protein Kinase C / genetics
  • Protein Kinase C / physiology*
  • Protein-Serine-Threonine Kinases
  • Proteins / metabolism
  • Synaptic Transmission / genetics

Substances

  • Caenorhabditis elegans Proteins
  • Carrier Proteins
  • Drosophila Proteins
  • Intracellular Signaling Peptides and Proteins
  • Microtubule-Associated Proteins
  • Nerve Growth Factors
  • Proteins
  • baz protein, Drosophila
  • futsch protein, Drosophila
  • par-6 protein, C elegans
  • Glutamic Acid
  • PAR-3 protein, C elegans
  • Protein-Serine-Threonine Kinases
  • PKC-3 protein
  • Protein Kinase C