Expression of KiSS-1, a metastasis suppressor gene, in trophoblast giant cells of the rat placenta

Biochim Biophys Acta. 2004 May 25;1678(2-3):102-10. doi: 10.1016/j.bbaexp.2004.02.005.


Metastin is encoded by a putative human metastasis suppressor gene KiSS-1, and is the cognate ligand of a G-protein-coupled receptor designated OT7T175. To study the physiological function(s) of metastin, we cloned rat and mouse KiSS-1 cDNAs both encoding 130-amino acid KiSS-1 proteins. Sequence analysis suggested that processing of the rat and mouse KiSS-1 proteins produces 52-amino-acid peptides, each with an amidated carboxyl terminal and with a single possible disulfide bond, corresponding to rat and mouse metastins. The carboxyl-terminal sequence of metastin, known to be essential for functional receptor interaction, was found to be highly conserved among humans and rodents. Real-time PCR analysis indicated that rat KiSS-1 mRNA showed the highest expression level in the cecum and colon. Since KiSS-1 mRNA and metastin are known to be abundant in human placenta, we further studied the localization of KiSS-1 and OT7T175 mRNAs in rat placenta by in situ hybridization. KiSS-1 and OT7T175 mRNAs were specifically detected in trophoblast giant cells at embryonic day 12.5, and the transcripts in the cells gradually decreased during placental maturation. These results suggest that metastin/OT7T175 signaling may participate in implantation of the mammalian embryo, placenta formation, and maintenance of pregnancy.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • CHO Cells
  • Cloning, Molecular
  • Cricetinae
  • DNA, Complementary / metabolism
  • Disulfides
  • Dose-Response Relationship, Drug
  • Embryo Implantation
  • Embryo, Mammalian / metabolism
  • Genes, Tumor Suppressor*
  • Giant Cells / metabolism*
  • In Situ Hybridization
  • Kisspeptins
  • Mice
  • Molecular Sequence Data
  • Neoplasm Metastasis
  • Peptides / chemistry
  • Placenta / metabolism*
  • Protein Biosynthesis*
  • Proteins / genetics
  • Proteins / physiology
  • RNA, Messenger / metabolism
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Time Factors
  • Tissue Distribution
  • Trophoblasts / metabolism


  • DNA, Complementary
  • Disulfides
  • Kiss1 protein, mouse
  • Kiss1 protein, rat
  • Kisspeptins
  • Peptides
  • Proteins
  • RNA, Messenger

Associated data

  • GENBANK/AY182231
  • GENBANK/AY196983