Selective inhibition of glial cell metabolism in vivo by fluorocitrate

Brain Res. 1992 Mar 27;576(1):120-4. doi: 10.1016/0006-8993(92)90616-h.


The effect of fluorocitrate on glial and neuronal amino acid metabolism was studied. One nmol of fluorocitrate administered intrastriatally in the rat caused a 95% reduction of glutamine formation from [14C]acetate, a substrate which enters the glial cells selectively. The metabolism of [14C]glucose which enters neurons, was unaffected by fluorocitrate treatment except for the glutamine formation. This is evidence that fluorocitrate is a selective inhibitor of the glial Krebs' cycle. [14C]Citrate and 2-oxoglutarate labelled amino acids in a manner similar to [14C]acetate, which shows that these substrates are taken up and metabolized by glial cells. Differences in the labelling of gamma-aminobutyric acid (GABA) from [14C]acetate and citrate suggest that astrocytes associated with GABAergic and glutamatergic nerve terminals may differ in their preference for amino acid precursors.

MeSH terms

  • Acetates / metabolism
  • Animals
  • Aspartic Acid / metabolism
  • Carbon Radioisotopes
  • Citrates / administration & dosage
  • Citrates / metabolism
  • Citrates / pharmacology*
  • Corpus Striatum / drug effects
  • Corpus Striatum / metabolism*
  • Glutamates / metabolism
  • Glutamine / metabolism
  • Ketoglutaric Acids / metabolism
  • Male
  • Neuroglia / drug effects
  • Neuroglia / metabolism*
  • Radioisotope Dilution Technique
  • Rats
  • Rats, Inbred Strains
  • Reference Values
  • Stereotaxic Techniques
  • gamma-Aminobutyric Acid / metabolism


  • Acetates
  • Carbon Radioisotopes
  • Citrates
  • Glutamates
  • Ketoglutaric Acids
  • Glutamine
  • Aspartic Acid
  • fluorocitrate
  • gamma-Aminobutyric Acid