Chromosome damaging effects of pan masala

Cancer Lett. 1992 Aug 31;65(3):221-6. doi: 10.1016/0304-3835(92)90235-n.


Effects of aqueous extracts of a popular brand of pan masala with and without tobacco (PM-T and PM) were studied for short duration treatment employing an in vitro system. Metabolic activation with S9 mix was also included. Frequency of all the three cytogenetic endpoints viz., chromosome aberration (CA); sister chromatid exchange (SCE) and % micronucleated cells (% MNC) were found to be elevated significantly in a dose-dependent manner in cultures without metabolic activation. However, addition of S9 activation system resulted in suppression of chromosomal damage. Our findings indicate that pan masalas contain water soluble direct acting mutagens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Areca*
  • CHO Cells / drug effects
  • Chromosome Aberrations*
  • Cricetinae
  • Cricetulus
  • Enzyme Activation
  • Liver / enzymology
  • Liver Extracts
  • Micronucleus Tests
  • Mutagenicity Tests
  • Mutagens*
  • Plant Extracts / toxicity*
  • Plants, Medicinal*
  • Sister Chromatid Exchange*


  • Liver Extracts
  • Mutagens
  • Plant Extracts