Eight Gram-Negative Bacteria Are 10,000 Times More Sensitive to Cationic Detergents Than to Anionic Detergents

Can J Microbiol. 2003 Dec;49(12):775-9. doi: 10.1139/w03-100.

Abstract

In liquid culture, eight typical gram-negative bacteria were ca. 10,000-fold more sensitive to cationic detergents than to the anionic detergent sodium dodecyl sulfate. Cetyltrimethylammonium bromide (CTAB) was inhibitory at concentrations ranging from 0.0006% to 0.01%. Four pseudomonads able to form biofilms were ca. 1000-fold more resistant to CTAB on Luria-Bertani agar plates than they were in liquid culture. A lasI mutant of Pseudomonas aeruginosa was only able to tolerate 0.1% CTAB on Luria-Bertani agar plates but could tolerate 5% CTAB when supplemented with homoserine lactone containing culture supernatants.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • 4-Butyrolactone / analogs & derivatives
  • 4-Butyrolactone / metabolism
  • Anions
  • Benzalkonium Compounds / pharmacology
  • Cations
  • Cetrimonium
  • Cetrimonium Compounds / pharmacology*
  • Cetylpyridinium / pharmacology
  • Detergents / pharmacology*
  • Enterobacter cloacae / drug effects
  • Enterobacter cloacae / growth & development
  • Escherichia coli / drug effects
  • Escherichia coli / growth & development
  • Gram-Negative Bacteria / drug effects*
  • Gram-Negative Bacteria / growth & development
  • Pseudomonas / drug effects
  • Pseudomonas / genetics
  • Pseudomonas / growth & development
  • Pseudomonas / metabolism
  • Serratia marcescens / drug effects
  • Serratia marcescens / growth & development
  • Sodium Dodecyl Sulfate / pharmacology

Substances

  • Anions
  • Benzalkonium Compounds
  • Cations
  • Cetrimonium Compounds
  • Detergents
  • homoserine lactone
  • Sodium Dodecyl Sulfate
  • Cetylpyridinium
  • 4-Butyrolactone
  • Cetrimonium