Recognition of HLA-A3 and HLA-A11 by KIR3DL2 is peptide-specific

Eur J Immunol. 2004 Jun;34(6):1673-9. doi: 10.1002/eji.200425089.


The recognition of MHC class I molecules by killer cell immunoglobulin-like receptors (KIR) is central to the control of NK cell function and can also modulate the CTL activation threshold. Among KIR receptors, KIR3DL2 is thought to interact with HLA-A3 and -A11, although direct evidence has been lacking. In this study, we show that HLA-A3 and -A11 tetramers specifically bind to KIR3DL2*001 transfectants and that this recognition is peptide-specific. Single amino acid substitutions in the nonamer peptide underline a critical role for residue 8 in recognition of KIR3DL2. However, the role of this interaction in vivo still remains to be established.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Flow Cytometry
  • HLA-A Antigens / immunology*
  • HLA-A11 Antigen
  • HLA-A3 Antigen / immunology*
  • Humans
  • Killer Cells, Natural / immunology
  • Peptide Fragments
  • Protein Conformation
  • Receptors, Immunologic / immunology*
  • Receptors, KIR
  • Receptors, KIR3DL2
  • T-Lymphocytes, Cytotoxic / immunology
  • Transfection


  • HLA-A Antigens
  • HLA-A11 Antigen
  • HLA-A3 Antigen
  • KIR3DL2 protein, human
  • Peptide Fragments
  • Receptors, Immunologic
  • Receptors, KIR
  • Receptors, KIR3DL2