Cell-free Protein Synthesis for Proteomics

Brief Funct Genomic Proteomic. 2004 Feb;2(4):308-19. doi: 10.1093/bfgp/2.4.308.


The use of cell-free expression systems as an alternative to cell-based methods for protein production is greatly facilitating studies of protein functions. Recent improvements to cell-free systems, and the development of cell-free protein display and microarray technologies, have led to cell-free protein synthesis becoming a powerful tool for large-scale analysis of proteins. This paper reviews the most commonly used cell-free systems and their applications in proteomics.

Publication types

  • Review

MeSH terms

  • Animals
  • Cell-Free System*
  • Genome
  • Humans
  • Mutagenesis, Site-Directed
  • Mutation
  • Protein Array Analysis / methods*
  • Protein Folding
  • Proteome
  • Proteomics / instrumentation*
  • Proteomics / methods*
  • RNA, Messenger / metabolism
  • Ribosomes / metabolism


  • Proteome
  • RNA, Messenger