Combining different 'omics' technologies to map and validate protein-protein interactions in humans

Brief Funct Genomic Proteomic. 2004 Feb;2(4):357-65. doi: 10.1093/bfgp/2.4.357.


The mapping of protein-protein interactions is key to understanding biological processes. Many technologies have been reported to map interactions and these have been systematically applied in yeast. To date, the number of reported yeast protein interactions that have been truly validated by at least one other approach is low. The mapping of human protein interaction networks is even more complicated. Thus, it is unreasonable to try to map the human interactome; instead, interaction mapping in human cell lines should be focused along the lines of diseases or changes that can be associated with specific cells. In this paper, an approach for combining different 'omics' technologies to achieve efficient mapping and validation of protein interactions in human cell lines is presented.

Publication types

  • Review

MeSH terms

  • Cell Line
  • Chromatography / methods
  • Chromosome Mapping
  • DNA, Complementary / metabolism
  • Fungal Proteins / metabolism
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Humans
  • Luminescent Proteins / metabolism
  • Protein Binding*
  • Proteomics / methods*
  • Two-Hybrid System Techniques


  • DNA, Complementary
  • Fungal Proteins
  • Luminescent Proteins
  • Green Fluorescent Proteins