Protein kinase C beta is dispensable for TCR-signaling

Mol Immunol. 2004 Jun;41(4):385-90. doi: 10.1016/j.molimm.2004.03.007.


PKCbeta has been established to be essential in B cell receptor (BCR) signaling. Additionally, a critical role of PKCbeta in TCR/CD28-stimulated regulation of IL-2 gene transcription but also exocytotic IL-2 secretion was observed in leukemic T cell lines. To now study the physiological function of PKCbeta in primary CD3(+) T cells, we used our established PKCbeta null mice. Unexpectantly, we did not reveal any defect in the development and function of T cells. Proliferative responses as well as IL-2 cytokine secretion of PKCbeta-deficient CD3(+) T cells induced by allogenic MHC, plate-bound anti-CD3 antibodies (with or without anti-CD28 costimulation), or mitogenic stimuli such as phorbol ester and Ca(2+) ionophore were comparable with wild-type controls. Thus, PKCbeta-deficient T cells had similar physiological thresholds for activation in vitro. These findings suggest that PKCbeta plays a redundant role in TCR-induced regulation of IL-2 cytokine production and T cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD28 Antigens / immunology
  • Cell Division
  • Genes, Reporter
  • Humans
  • Interleukin-2 / genetics
  • Interleukin-2 / metabolism
  • Ionomycin / pharmacology
  • Ionophores / pharmacology
  • Isoenzymes / genetics
  • Isoenzymes / physiology
  • Jurkat Cells
  • Luciferases / biosynthesis
  • Luciferases / genetics
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred BALB C
  • Mice, Knockout
  • Muromonab-CD3 / pharmacology
  • Phorbol 12,13-Dibutyrate / pharmacology
  • Promoter Regions, Genetic / genetics
  • Protein Kinase C / deficiency
  • Protein Kinase C / genetics
  • Protein Kinase C / physiology*
  • Protein Kinase C-theta
  • Receptors, Antigen, T-Cell / physiology*
  • Recombinant Fusion Proteins / physiology
  • Signal Transduction
  • T-Lymphocytes / enzymology*
  • T-Lymphocytes / immunology
  • Transfection


  • CD28 Antigens
  • Interleukin-2
  • Ionophores
  • Isoenzymes
  • Muromonab-CD3
  • Receptors, Antigen, T-Cell
  • Recombinant Fusion Proteins
  • Phorbol 12,13-Dibutyrate
  • Ionomycin
  • Luciferases
  • PRKCQ protein, human
  • Prkcq protein, mouse
  • Protein Kinase C
  • Protein Kinase C-theta