Purified angiotensin converting enzyme from Rana esculenta ovary influences ovarian steroidogenesis in vitro

J Physiol Biochem. 2003 Dec;59(4):269-76. doi: 10.1007/BF03179884.


The aim of the present study was to purify and characterize angiotensin-converting enzyme (ACE) present in frog ovary (Rana esculenta). Detergent and trypsin-extracted enzymes were purified using a one-step process, consisting of affinity chromatography on lisinopril coupled to Sepharose 6B. The molecular mass was 150 kDa for both detergent-extracted and trypsin-extracted enzyme. The specific activity of detergent-extracted and trypsin-extracted ACE was 294 U mg(-1) and 326 U mg(-1) respectively. The optimum pH range was from 7-8.5 at 37 degrees C and the optimum temperature was 50 degrees C. Optimum chloride concentration was about 200 mM for synthetic substrate FAPGG (N-[3-(2-furyl)acryloyl] L-phenylalanyl glycyl glycine) and angiotensin I, and 10 mM for bradykinin. The Km and Kcat values for FAPGG were 0.608 +/- 0.07 mM and 249 sec(-1) respectively and I50 values for captopril and lisinopril, two specific ACE inhibitors, were 68 +/- 12.55 nM and 6.763 +/- 0.66 nM respectively. Frog ovary tissue from prereproductive period was incubated in vitro in the presence of frog ovary ACE (2.5 mU/ml), captopril (0.1 mM), and lisinopril (0.1 mM). Production of 17beta-estradiol, progesterone, and prostaglandins E2 and F2alpha was determined. The data showed a modulation of 17beta-estradiol, progesterone and prostaglandin E2 production by ovary ACE.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angiotensin I / metabolism
  • Angiotensin-Converting Enzyme Inhibitors / metabolism
  • Animals
  • Bradykinin / metabolism
  • Captopril / metabolism
  • Female
  • Hydrogen-Ion Concentration
  • Lisinopril / metabolism
  • Oligopeptides / metabolism
  • Ovary / enzymology*
  • Rana esculenta / anatomy & histology
  • Rana esculenta / metabolism*
  • Renin / isolation & purification*
  • Renin / metabolism
  • Sodium Chloride / metabolism
  • Steroids / biosynthesis*
  • Temperature


  • Angiotensin-Converting Enzyme Inhibitors
  • Oligopeptides
  • Steroids
  • Sodium Chloride
  • 2-furanacryloyl-phenylalanyl-glycyl-glycine
  • Angiotensin I
  • Captopril
  • Lisinopril
  • Renin
  • Bradykinin