Possible influence of infrasound on glioma cell response to chemotherapy: a pilot study

J Altern Complement Med. 2004 Apr;10(2):247-50. doi: 10.1089/107555304323062239.


Objective: To assess the response of cultured human tumor cells to infrasound in combination with conventional anticancer agents using an infrasound-emitting apparatus marketed as a therapeutic device.

Design: Two pilot experiments measured proliferation of cultured brain tumor cells exposed to three treatment conditions: infrasound emission alone, infrasound in combination with the chemotherapy 5-fluorouracil (5-FU), and infrasound in combination with ionizing radiation. Results from each experimental condition were compared to those from appropriate control conditions.

Outcome measure: A standard colony-forming efficiency assay was used to assess tumor cell proliferation.

Results: Tumor cell proliferation was not significantly altered by treatment with infrasound alone, nor did infrasound appear to influence cellular response to x-rays. There was a significant interaction between 5-FU and infrasound (P < 0.0001), however, evident in decreased colony formation.

Conclusion: Further research is warranted to assess potential synergism between infrasound and 5-FU against tumor cell proliferation, and to investigate the possible therapeutic use of infrasound.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antimetabolites, Antineoplastic / therapeutic use*
  • Brain Neoplasms / drug therapy
  • Brain Neoplasms / radiotherapy
  • Brain Neoplasms / therapy*
  • Cell Division
  • Cell Line, Tumor
  • Combined Modality Therapy
  • Fluorouracil / therapeutic use*
  • Glioblastoma / drug therapy
  • Glioblastoma / radiotherapy
  • Glioblastoma / therapy*
  • Humans
  • Pilot Projects
  • Radiation, Ionizing*
  • Time Factors
  • Tumor Cells, Cultured
  • Ultrasonic Therapy*


  • Antimetabolites, Antineoplastic
  • Fluorouracil