Peripheral leptin administration alters hormone and metabolite levels in the young pig

Comp Biochem Physiol A Mol Integr Physiol. 2004 May;138(1):17-25. doi: 10.1016/j.cbpb.2004.02.005.


The present study was conducted to determine if peripheral leptin administration can alter GH secretion or feed intake in young pigs. Six, 6 kg female pigs were fasted overnight and randomly chosen to receive porcine recombinant leptin or saline injections in a crossover design. Three leptin dosages were tested over a 10 day period, 100, 200 or 500 microg/kg body mass (L100, L200 or L500). Leptin was administered in 0.2% bovine serum albumin as a bolus injection into the carotid artery. Blood samples were obtained from the jugular vein over a 24 h period. Leptin delayed feeding in pigs treated with L200 and L500 (P<0.05), while reducing overall intake in pigs treated with L100 (P<0.05). L200 or L500 depressed blood glucose (P<0.05). Plasma insulin levels were elevated by feeding in control animals, while insulin levels were depressed in pigs treated with L200 or L500 (P<0.05). L200 elevated plasma growth hormone (P<0.05) with three peaks apparent at 5, 8, and 13 h post injection. The ability for a single injection of leptin to produce significant changes in hormone and metabolite levels suggests that this peptide has a role in regulation of peripheral metabolism.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Blood Glucose / analysis*
  • Cross-Over Studies
  • Dose-Response Relationship, Drug
  • Eating / drug effects
  • Fatty Acids, Nonesterified / blood*
  • Female
  • Growth Hormone / blood*
  • Insulin / blood
  • Leptin / administration & dosage*
  • Leptin / pharmacology
  • Swine


  • Blood Glucose
  • Fatty Acids, Nonesterified
  • Insulin
  • Leptin
  • Growth Hormone