Influenza virus A infection induces interleukin-8 gene expression in human airway epithelial cells

FEBS Lett. 1992 Sep 14;309(3):327-9. doi: 10.1016/0014-5793(92)80799-m.


To determine the role of the airway epithelial cell in mediating virus-induced inflammation, we infected primary cultures of human airway epithelial cells with human influenza type A/Port Chalmers/72 (H3N2). After two days, the medium was collected for measurement of the chemotactic cytokine interleukin-8 by enzyme-linked immunosorbent assay. The RNA was extracted from the cells for analysis of interleukin-8 mRNA by Northern blot analysis. Interleukin-8 production was more than doubled by viral infection, while interleukin-8 mRNA was increased four-fold. Thus induction of interleukin-8 gene expression in virus-infected airway epithelium may be an important early step leading to virus-induced airway inflammation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Cells, Cultured
  • DNA
  • Epithelial Cells
  • Epithelium / metabolism
  • Epithelium / microbiology
  • Gene Expression Regulation*
  • Humans
  • Influenza A virus / physiology*
  • Interleukin-8 / genetics*
  • Interleukin-8 / metabolism
  • Lung / cytology
  • Lung / metabolism
  • Lung / microbiology*
  • Molecular Sequence Data
  • Virus Replication


  • Interleukin-8
  • DNA