Molecular cloning and expression of the human melanocyte stimulating hormone receptor cDNA

FEBS Lett. 1992 Sep 14;309(3):417-20. doi: 10.1016/0014-5793(92)80820-7.


Melanocytes and melanoma cells are known to possess receptors for melanocyte stimulating hormone (MSH). A cDNA clone, designated 11D, has been isolated from human melanoma cells and encodes a MSH receptor. The cloned cDNA encodes a 317 amino acid protein with transmembrane topography characteristics of a G-protein-coupled receptor, but it does not show striking similarity to already published sequences of other G-protein-coupled receptors. When 11D cDNA is expressed in COS-7 cells, it binds an 125I-labelled MSH analogue (NDP-MSH) in a specific manner. The bound ligand could be displaced by melanotropic peptides, alpha-MSH, beta-MSH, gamma-MSH and ACTH (adrenocorticotropic hormone), but not by the non-melanotropic peptide, beta-endorphin. This is the first report of the cloning of the receptor gene of the melanotropin receptor family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Cloning, Molecular
  • DNA
  • Humans
  • Melanocyte-Stimulating Hormones / metabolism*
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Receptors, Pituitary Hormone / genetics*
  • Receptors, Pituitary Hormone / metabolism


  • Receptors, Pituitary Hormone
  • MSH receptor
  • Melanocyte-Stimulating Hormones
  • DNA

Associated data

  • GENBANK/S39995
  • GENBANK/S39996
  • GENBANK/S40001
  • GENBANK/S40008
  • GENBANK/S40009
  • GENBANK/S40010
  • GENBANK/X66432
  • GENBANK/X67594
  • GENBANK/X70911
  • GENBANK/X70912