Previously, we reported the purification of a 29-kDa proteinase from the pupal hemocytes of Sarcophaga peregrina (flesh fly). Antibody against this proteinase was found to inhibit dissociation of the fat body by pupal hemocytes in vitro. Moreover, the purified enzyme alone was shown to dissociate the fat body. This enzyme was found to be localized in granules of heterogeneous size in the hemocytes and to be released on their interaction with the fat body. From these results, we conclude that this 29-kDa proteinase participates in dissociation of the fat body at metamorphosis.