Abstract
We examined the effects of EPO on expression of suppressor of cytokine signaling 2 (SOCS2) and found that treatment of neural progenitor cells derived from the adult subventricular zone (SVZ) with recombinant human EPO (rhEPO) stimulated progenitor cell differentiation into neurons, but not astrocytes. Quantitative RT-PCR revealed that SOCS2 mRNA levels were increased in the progenitor cells treated with rhEPO. Immunostaining showed that neurons but not astrocytes were SOCS2 immunoreactive. Incubation of the progenitor cells with rhEPO in the presence of a neutralizing antibody against EPO abolished the effects of EPO on neuronal differentiation and expression of SOCS2. Our data suggest that up-regulation of SOCS2 in neuronal progenitor cells derived from the adult SVZ may regulate EPO enhanced neuronal differentiation.
Copyright 2004 Lippincott Williams and Wilkins
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies / pharmacology
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Astrocytes / cytology
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Astrocytes / drug effects
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Astrocytes / metabolism
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Brain / cytology
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Brain / growth & development*
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Brain / metabolism
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Cell Differentiation / drug effects
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Cell Differentiation / physiology*
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Cells, Cultured
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DNA-Binding Proteins / genetics*
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Erythropoietin / antagonists & inhibitors
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Erythropoietin / metabolism*
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Immunohistochemistry
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Lateral Ventricles / cytology
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Lateral Ventricles / growth & development
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Male
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Neurons / cytology
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Neurons / drug effects
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Neurons / metabolism*
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RNA, Messenger / drug effects
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RNA, Messenger / metabolism
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Rats
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Rats, Wistar
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Repressor Proteins / genetics*
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Spheroids, Cellular / cytology
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Spheroids, Cellular / drug effects
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Spheroids, Cellular / metabolism
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Stem Cells / cytology
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Stem Cells / drug effects
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Stem Cells / metabolism*
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Suppressor of Cytokine Signaling Proteins
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Trans-Activators / genetics*
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Up-Regulation / drug effects
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Up-Regulation / physiology
Substances
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Antibodies
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DNA-Binding Proteins
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RNA, Messenger
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Repressor Proteins
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Socs2 protein, rat
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Suppressor of Cytokine Signaling Proteins
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Trans-Activators
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Erythropoietin