Coenzyme Q10 and lipid-related gene induction in HeLa cells

Am J Obstet Gynecol. 2004 May;190(5):1432-4. doi: 10.1016/j.ajog.2004.01.076.

Abstract

Objective: Coenzyme Q10 is an antioxidant that may have a therapeutic role in cervical cancer.

Study design: We investigated the cellular and molecular effects of 30 micromol/L Coenzyme Q10 in HeLa cells. Cell growth assays, fluorescence-activated cell sorting analyses, and Oil Red O staining were performed. Microarray experiments were performed in duplicate and analyzed on the basis of 2-fold changes in levels of gene expression.

Results: Coenzyme Q10 inhibited cell growth and led to apoptosis. Microarray analysis showed that 264 sequences were altered over time, with enrichment in lipid-related genes. Enhanced lipid accumulation was confirmed with Oil Red O staining.

Conclusion: A lipid response to Coenzyme Q10 may affect mechanisms of growth inhibition in HeLa cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antioxidants / pharmacology*
  • Apoptosis / drug effects*
  • Apoptosis / physiology
  • Cell Division / drug effects*
  • Coenzymes
  • Female
  • Gene Expression Regulation, Neoplastic
  • HeLa Cells / drug effects
  • Humans
  • Lipid Peroxidation
  • Oxidation-Reduction
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • Reference Values
  • Sensitivity and Specificity
  • Transcriptional Activation
  • Ubiquinone / analogs & derivatives*
  • Ubiquinone / pharmacology*
  • Uterine Cervical Neoplasms

Substances

  • Antioxidants
  • Coenzymes
  • RNA, Messenger
  • Ubiquinone
  • coenzyme Q10