Cloning and Characterization of a Novel Human TGF-beta Activated Kinase-Like Gene

Biochem Genet. 2004 Apr;42(3-4):129-37. doi: 10.1023/b:bigi.0000020468.96238.54.

Abstract

TGF-beta activated kinase (TAK1) plays a critical role in the TGF-beta signaling transduction pathway. By screening a human 18-week fetal brain library, we isolated a novel human TAK1-like (TAKL) gene. The gene encoded a putative protein of 242 amino acids, which shared a homology with human, mouse, and Xenopus TAK1. The TAKL gene was located in chromosome 21q21. Northern blot analysis revealed that the TAKL mRNA was expressed predominantly in peripheral blood leukocytes and ubiquitously in human adult and fetal tissues. TAKL was also expressed strongly in breast carcinoma GI-101, colon adenocarcinoma GI-112, and prostatic adenocarcinoma PC3.

MeSH terms

  • Adenocarcinoma / genetics
  • Amino Acid Sequence
  • Base Sequence
  • Breast Neoplasms / genetics
  • Carcinoma / genetics
  • Chromosomes, Human, Pair 21
  • Cloning, Molecular*
  • Colonic Neoplasms / genetics
  • Exons
  • Female
  • Fetus
  • Humans
  • Introns
  • Leukocytes, Mononuclear / chemistry
  • MAP Kinase Kinase Kinases / chemistry
  • MAP Kinase Kinase Kinases / genetics*
  • MAP Kinase Kinase Kinases / isolation & purification*
  • Male
  • Molecular Sequence Data
  • Open Reading Frames
  • Pregnancy
  • Prostatic Neoplasms / genetics
  • Protein Kinases / chemistry
  • Protein Kinases / genetics*
  • Protein Kinases / isolation & purification*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • RNA, Messenger
  • Protein Kinases
  • MAP3K7CL protein, human
  • MAP Kinase Kinase Kinases
  • MAP kinase kinase kinase 7

Associated data

  • GENBANK/AY171599