Lentivirally generated eGFP-transgenic rats allow efficient cell tracking in vivo

Genesis. 2004 Jun;39(2):94-9. doi: 10.1002/gene.20037.

Abstract

Here we describe the efficient generation of eGFP-transgenic rats using a lentiviral approach. Analysis of the founder generation demonstrated that 46% of the offspring had stably integrated the provirus into the genome and of those 92% expressed eGFP in all blood-derived leukocytes. In contrast to their offspring, all founder rats were mosaic with regard to eGFP-expression, suggesting delayed viral transduction after injection. The expression level of eGFP in the F1 generation is influenced by and segregates with the site of proviral integration. Interestingly, a single copy of the transgene is sufficient for reliable detection by flow cytometry, irrespective of the leukocyte subtype analyzed. Adoptive transfer of purified CD4(+) T-lymphocytes from transgenic rats and subsequent reisolation from various organs further demonstrated that expression of the lentiviral transgene is maintained in a foreign host and therefore allows for efficient tracking of transferred cells. Taken together, lentivirally generated eGFP-transgenic rats are a powerful tool for various applications in immunology and presumably also many other fields.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Animals, Genetically Modified / metabolism*
  • CD4-Positive T-Lymphocytes / metabolism
  • Female
  • Flow Cytometry
  • Gene Transfer Techniques
  • Genetic Vectors
  • Green Fluorescent Proteins / metabolism*
  • Lentivirus / genetics*
  • Leukocytes / metabolism
  • Male
  • Rats
  • Rats, Inbred Lew
  • Rats, Sprague-Dawley
  • Transduction, Genetic
  • Transgenes / physiology

Substances

  • enhanced green fluorescent protein
  • Green Fluorescent Proteins