Hepatitis B virus X protein induces expression of Fas ligand gene through enhancing transcriptional activity of early growth response factor

J Biol Chem. 2004 Aug 27;279(35):36242-9. doi: 10.1074/jbc.M401290200. Epub 2004 Jun 1.

Abstract

FasL expressed in tumor cells plays an important role in the escape from immune surveillance by inducing apoptosis in T-cells bearing Fas. Since the Fas/FasL signaling pathway requires transcriptional induction of the FasL gene, elucidation of the precise mechanisms underlying regulation of FasL gene expression may provide useful molecular insights on tumor progression. We and others (Shin, E. C., Shin, J. S., Park, J. H., Kim, H., and Kim, S. J. (1999) Int. J. Cancer 82, 587-591; Lee, M. O., Kang, H. J., Cho, H., Shin, E. C., Park, J. H., and Kim, S. J. (2001) Biochem. Biophys. Res. Commun. 288, 1162-1168) have previously reported that hepatitis B virus X protein (HBx) plays a role in the induction of FasL expression in hepatitis B virus-associated hepatoma. In the present study, we analyzed the potential cis- and trans-acting factors that regulate FasL promoter. We found that HBx induced activity of the reporter containing FasL promoter through binding site for Egr but not through NFAT or SP-1, which are known as strong activators of the FasL promoter in T-cells. Transient expression of antisense Egr-2 and antisense Egr-3 abolished expression of FasL, which further confirmed the role of Egr in the HBx-mediated FasL expression. Also we observed that HBx increased the transcriptional activity of Egr-2 and Egr-3 by enhancing expression as well as the transactivation function of these proteins. HBx interacted with Egr-2 and Egr-3 in vivo and enhanced binding of Egr to the co-activator, cAMP-response element-binding protein-binding protein, which may explain the molecular mechanism by which HBx induced the transactivation function of Egr. Finally, we found that the carboxyl terminus of HBx was necessary and sufficient for FasL induction as well as activation of Egr. Taken together, our results show a novel mechanism by which HBx induces FasL gene expression that is mediated by enhancing transcriptional activity of Egr-2 and Egr-3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis
  • Base Sequence
  • Binding Sites
  • Blotting, Western
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / pathology
  • Cell Line
  • Cell Line, Tumor
  • DNA-Binding Proteins / metabolism*
  • Disease Progression
  • Early Growth Response Protein 2
  • Early Growth Response Protein 3
  • Fas Ligand Protein
  • Gene Expression Regulation
  • Genes, Reporter
  • Humans
  • Liver Neoplasms / pathology
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Molecular Sequence Data
  • NIH 3T3 Cells
  • Oligonucleotides, Antisense / pharmacology
  • Plasmids / metabolism
  • Precipitin Tests
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Structure, Tertiary
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • T-Lymphocytes / metabolism
  • Trans-Activators / metabolism*
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Transcriptional Activation
  • Transfection
  • Up-Regulation
  • Viral Regulatory and Accessory Proteins
  • fas Receptor / metabolism

Substances

  • DNA-Binding Proteins
  • EGR2 protein, human
  • EGR3 protein, human
  • Early Growth Response Protein 2
  • Egr2 protein, mouse
  • FASLG protein, human
  • Fas Ligand Protein
  • Fasl protein, mouse
  • Membrane Glycoproteins
  • Oligonucleotides, Antisense
  • Trans-Activators
  • Transcription Factors
  • Viral Regulatory and Accessory Proteins
  • fas Receptor
  • hepatitis B virus X protein
  • Early Growth Response Protein 3