Consequences of a deletion in dspA on transcript accumulation in Synechocystis sp. strain PCC6803

J Bacteriol. 2004 Jun;186(12):3889-902. doi: 10.1128/JB.186.12.3889-3902.2004.

Abstract

A sensor histidine kinase of Synechococcus sp. strain PCC7942, designated nblS, was previously identified and shown to be critical for the acclimation of cells to high-light and nutrient limitation conditions and to influence the expression of a number of light-responsive genes. The nblS orthologue in Synechocystis sp. strain PCC6803 is designated dspA (also called hik33). We have generated a dspA null mutant and analyzed global gene expression in both the mutant and wild-type strains under high- and low-light conditions. The mutant is aberrant for the expression of many genes encoding proteins critical for photosynthesis, phosphate and carbon acquisition, and the amelioration of stress conditions. Furthermore, transcripts from a number of genes normally detected only during exposure of wild-type cells to high-light conditions become partially constitutive in the low-light-grown dspA mutant. Other genes for which transcripts decline upon exposure of wild-type cells to high light are already lower in the mutant during growth in low light. These results suggest that DspA may influence gene expression in both a positive and a negative manner and that the dspA mutant behaves as if it were experiencing stress conditions (e.g., high-light exposure) even when maintained at near-optimal growth conditions for wild-type cells. This is discussed with respect to the importance of DspA for regulating the responses of the cell to environmental cues.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Cyanobacteria / genetics
  • Cyanobacteria / growth & development*
  • Cyanobacteria / metabolism
  • Gene Deletion*
  • Gene Expression Regulation, Bacterial*
  • Heat-Shock Response
  • Histidine Kinase
  • Light
  • Oligonucleotide Array Sequence Analysis
  • Photosynthesis
  • Protein Kinases / genetics
  • Protein Kinases / metabolism*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • Transcription, Genetic*

Substances

  • Bacterial Proteins
  • Protein Kinases
  • Histidine Kinase