A mutant allele of rpoD results in increased conversion of aminoimidazole ribotide to hydroxymethyl pyrimidine in Salmonella enterica

J Bacteriol. 2004 Jun;186(12):4034-7. doi: 10.1128/JB.186.12.4034-4037.2004.


An allele of rpoD (rpoD1181) that results in increased synthesis of the pyrimidine moiety of thiamine in Salmonella enterica was identified. The S508Y substitution caused by rpoD1181 is analogous to the S506F derivative of the Escherichia coli protein. The properties of this E. coli mutant protein have been well characterized in vitro. Identification of a metabolic phenotype caused by the rpoD1181 allele of S. enterica allows past in vitro results to be incorporated in continuing efforts to understand cellular processes that are integrated with the thiamine biosynthetic pathway.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alleles*
  • DNA-Directed RNA Polymerases / genetics*
  • Gene Expression Regulation, Bacterial*
  • Mutation*
  • Pyrimidines / metabolism*
  • Ribonucleotides / metabolism*
  • Salmonella enterica / genetics
  • Salmonella enterica / metabolism*
  • Sigma Factor / genetics*
  • Thiamine / biosynthesis
  • Transcription, Genetic


  • Pyrimidines
  • Ribonucleotides
  • Sigma Factor
  • aminoimidazole ribotide
  • RNA polymerase sigma 70
  • DNA-Directed RNA Polymerases
  • pyrimidine
  • Thiamine