In vivo and in vitro anti-inflammatory activity of Mangifera indica L. extract (VIMANG)

Pharmacol Res. 2004 Aug;50(2):143-9. doi: 10.1016/j.phrs.2003.12.003.

Abstract

A standard aqueous extract of Mangifera indica L., used in Cuba as an antioxidant under the brand name of VIMANG, was tested in vivo for its anti-inflammatory activity using commonly accepted assays. M. indica extract, administered topically (0.5-2 mg per ear), reduced ear edema induced by arachidonic acid (AA) and phorbol myristate acetate (PMA, ED50 = 1.1 mg per ear) in mice. In the PMA model, M. indica extract also reduced myeloperoxidase (MPO) activity. This extract p.o. administered also inhibited tumor necrosis factor alpha (TNFalpha) serum levels in both models of inflammation (AA, ED50 = 106.1 mg kg(-1) and PMA, ED50 = 58.2 mg kg(-1)). In vitro studies were performed using the macrophage cell line RAW264.7 stimulated with pro-inflammatory stimuli (LPS-IFNgamma or the calcium ionophore A23187) to determine PGE2 or LTB4 release, respectively. The extract inhibited the induction of PGE2 with IC50 = 64.1 microg ml(-1) and LTB4 IC50 = 22.9 microg ml(-1). M. indica extract also inhibited human synovial secretory phospholipase (PL)A2 with IC 50 = 0.7 microg ml(-1). These results represent an important contribution to the elucidation of the mechanism involved in the anti-inflammatory and anti-nociceptive effects reported by the standard M. indica extract VIMANG.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Topical
  • Animals
  • Anti-Inflammatory Agents, Non-Steroidal / chemistry
  • Anti-Inflammatory Agents, Non-Steroidal / isolation & purification
  • Anti-Inflammatory Agents, Non-Steroidal / therapeutic use*
  • Arachidonic Acid / administration & dosage
  • Arachidonic Acid / adverse effects
  • Arachidonic Acid / antagonists & inhibitors
  • Calcimycin / pharmacology
  • Cuba
  • Dexamethasone / pharmacology
  • Dinoprostone / metabolism
  • Disease Models, Animal
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical / methods
  • Drug Therapy, Combination
  • Ear, External / drug effects
  • Ear, External / physiopathology
  • Edema / chemically induced
  • Edema / drug therapy
  • Eicosanoids / metabolism
  • Indomethacin / pharmacology
  • Interferon-gamma / metabolism
  • Interferon-gamma / pharmacology
  • Leukotriene B4 / metabolism
  • Lipopolysaccharides / metabolism
  • Lipopolysaccharides / pharmacology
  • Macrophages / cytology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Male
  • Mangifera / chemistry*
  • Mice
  • Oleanolic Acid / analogs & derivatives*
  • Oleanolic Acid / pharmacology
  • Peroxidase / adverse effects
  • Peroxidase / antagonists & inhibitors
  • Phospholipases A / antagonists & inhibitors
  • Phospholipases A / metabolism
  • Phytotherapy*
  • Plant Bark
  • Plant Extracts / chemistry*
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Plant Stems
  • Plants, Medicinal / chemistry
  • Tetradecanoylphorbol Acetate / administration & dosage
  • Tetradecanoylphorbol Acetate / adverse effects
  • Tetradecanoylphorbol Acetate / antagonists & inhibitors
  • Tumor Necrosis Factor-alpha / antagonists & inhibitors
  • Tumor Necrosis Factor-alpha / drug effects
  • Tumor Necrosis Factor-alpha / metabolism
  • Water
  • Xanthones / pharmacology

Substances

  • Anti-Inflammatory Agents, Non-Steroidal
  • Eicosanoids
  • Lipopolysaccharides
  • Mangifera indica extract
  • Plant Extracts
  • Tumor Necrosis Factor-alpha
  • Xanthones
  • petrosaspongiolide m
  • Water
  • Leukotriene B4
  • mangiferin
  • Arachidonic Acid
  • Calcimycin
  • Oleanolic Acid
  • Dexamethasone
  • Interferon-gamma
  • Peroxidase
  • Phospholipases A
  • Dinoprostone
  • Tetradecanoylphorbol Acetate
  • Indomethacin