Improved method for the isolation of RNA from (standing liquid cultures of) Streptomycetes

J Microbiol Methods. 2004 Jul;58(1):139-42. doi: 10.1016/j.mimet.2004.03.014.


Streptomycetes are complex soil bacteria capable of producing aerial reproductive mycelium and secondary metabolites. We observed novel phenomena such as an extended life cycle including flotation and anaerobiosis using standing liquid cultures. This paper describes an improved method for isolating good quality RNA from standing liquid cultures of S. coelicolor via excellent cell lysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Northern
  • Deoxyribonuclease I / chemistry
  • Guanidines / chemistry
  • Isothiocyanates / chemistry
  • Phenol / chemistry
  • RNA, Bacterial / isolation & purification*
  • Spores, Bacterial
  • Streptomyces / chemistry
  • Streptomyces / genetics*


  • Guanidines
  • Isothiocyanates
  • RNA, Bacterial
  • guanidine isothiocyanate
  • Phenol
  • Deoxyribonuclease I