NMR solution structure and position of transportan in neutral phospholipid bicelles

FEBS Lett. 2004 Jun 4;567(2-3):265-9. doi: 10.1016/j.febslet.2004.04.079.


Transportan is a chimeric cell-penetrating peptide constructed from the peptides galanin and mastoparan, which has the ability to internalize living cells carrying a hydrophilic load. In this study, we have determined the NMR solution structure and investigated the position of transportan in neutral bicelles. The structure revealed a well-defined alpha-helix in the C-terminal mastoparan part of the peptide and a weaker tendency to form an alpha-helix in the N-terminal domain. The position of the peptide in relation to the membrane, as studied by adding paramagnetic probes, shows that the peptide lies parallel to, and in the head-group region of the membrane surface. This result is supported by amide proton secondary chemical shifts.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amides / chemistry
  • Amino Acid Sequence
  • Circular Dichroism
  • Dimyristoylphosphatidylcholine / chemistry*
  • Drug Carriers / chemistry
  • Galanin
  • Glycerophosphates / chemistry
  • Lipid Bilayers / chemistry*
  • Micelles
  • Models, Molecular
  • Molecular Sequence Data
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Phospholipid Ethers / chemistry*
  • Phosphorylcholine / analogs & derivatives*
  • Phosphorylcholine / chemistry
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins / chemistry*
  • Spin Labels
  • Wasp Venoms


  • 1,2-dihexadecyl-sn-glycero-3-phosphocholine
  • Amides
  • Drug Carriers
  • Glycerophosphates
  • Lipid Bilayers
  • Micelles
  • Phospholipid Ethers
  • Recombinant Fusion Proteins
  • Spin Labels
  • Wasp Venoms
  • transportan
  • Phosphorylcholine
  • dodecylphosphocholine
  • Galanin
  • Dimyristoylphosphatidylcholine