Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510

Zhenqing Zhang; Guangli Yu; Huashi Guan; Xia Zhao; Yuguo Du; Xiaolu Jiang

doi:10.1016/j.carres.2004.03.010

Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibro sp. 510

Carbohydr Res. 2004 Jun 1;339(8):1475-81. doi: 10.1016/j.carres.2004.03.010.

Authors

Zhenqing Zhang¹, Guangli Yu, Huashi Guan, Xia Zhao, Yuguo Du, Xiaolu Jiang

Affiliation

¹ Institute of Marine Drug and Food, Ocean University of China, Qingdao 266003, China.

PMID: 15178390
DOI: 10.1016/j.carres.2004.03.010

Abstract

Alginate that was purified from the fermentation solution of marine bacteria Vibro sp. 510 under specific reaction conditions was hydrolyzed by alginate lyase. Seven oligosaccharides, including di-, tri- and tetrasaccharides, were isolated through low-pressure, gel-permeation chromatography (LP-GPC) and semipreparative strong-anion exchange (SAX) fast-protein liquid chromatography (FPLC). The oligosaccharide structures were elucidated based on ESIMS and 2D NMR spectral analysis. The hydrolytic specificity of this alginate lyase to alginate is discussed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alginates / chemistry*
Bacteria / enzymology*
Chromatography, High Pressure Liquid
Glucuronic Acid / chemistry*
Hexuronic Acids / chemistry*
Magnetic Resonance Spectroscopy
Oligosaccharides / chemistry*
Oligosaccharides / isolation & purification
Oligosaccharides / metabolism*
Polysaccharide-Lyases / metabolism*
Spectrometry, Mass, Electrospray Ionization

Substances

Alginates
Hexuronic Acids
Oligosaccharides
Glucuronic Acid
Polysaccharide-Lyases
poly(beta-D-mannuronate) lyase