Explant-cell culture of primary mammary tumors from MMTV-c-Myc transgenic mice

In Vitro Cell Dev Biol Anim. Jan-Feb 2004;40(1-2):14-21. doi: 10.1290/1543-706X(2004)40<14:ECOPMT>2.0.CO;2.

Abstract

We have established an explant-cell culture system for mammary gland tumors from c-myc oncogene-expressing transgenic mice and potentially other transgenic strains. By coating culture dish surfaces with fetal bovine serum and using culture media supplemented with low serum and growth factors, the mammary tumor specimens could be maintained in culture for over 3 mo. Throughout the culture period, the explants produced abundant outgrowths of epithelial cells. As the outgrowths of epithelial cells filled the dishes, the explants were serially transferred from one dish to another-a process that could be repeated at least six times, thus providing a continuous supply of primary tumor cells. This culture system provides a useful tool for studying the biology of mouse mammary gland tumors and possibly tumors from other organ sites.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Breast Neoplasms / genetics
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Cattle
  • Cell Culture Techniques*
  • Cell Shape
  • Culture Media / chemistry
  • Female
  • Humans
  • Keratins / metabolism
  • Mammary Tumor Virus, Mouse / genetics
  • Mammary Tumor Virus, Mouse / metabolism*
  • Mice
  • Mice, Transgenic*
  • Proto-Oncogene Proteins c-myc / genetics
  • Proto-Oncogene Proteins c-myc / metabolism*
  • Salivary Gland Neoplasms / metabolism
  • Salivary Gland Neoplasms / pathology
  • Tumor Cells, Cultured

Substances

  • Culture Media
  • Myc protein, mouse
  • Proto-Oncogene Proteins c-myc
  • Keratins