Use of paraffin-embedded tissue for identification of Saccharomyces cerevisiae in a baker's lung nodule by fungal PCR and nucleotide sequencing

Ping Ren; Sundara Sridhar; Vishnu Chaturvedi

doi:10.1128/JCM.42.6.2840-2842.2004

Use of paraffin-embedded tissue for identification of Saccharomyces cerevisiae in a baker's lung nodule by fungal PCR and nucleotide sequencing

J Clin Microbiol. 2004 Jun;42(6):2840-2. doi: 10.1128/JCM.42.6.2840-2842.2004.

Authors

Ping Ren¹, Sundara Sridhar, Vishnu Chaturvedi

Affiliation

¹ Mycology Laboratory, Wadsworth Center, New York State Department of Health, SUNY School of Public Health, University at Albany, Albany, NY 12201-2002, USA.

Abstract

A 40-year-old healthy male employed in a bakery presented with a single lung nodule and underwent investigations to rule out pulmonary carcinoma. Biopsy was positive for yeast cells, which did not match common fungal pathogens. PCR assay of paraffin-embedded tissue and nucleotide sequencing with ribosomal ITS1-ITS2 universal primers revealed the presence of Saccharomyces cerevisiae.

Publication types

Case Reports

MeSH terms

Adult
Base Sequence
Humans
Lung / microbiology*
Male
Molecular Sequence Data
Occupational Exposure
Paraffin Embedding
Polymerase Chain Reaction / methods*
Saccharomyces cerevisiae / isolation & purification*
Sequence Analysis, DNA

Associated data

GENBANK/AF500488
GENBANK/AY525600