[Activation of mouse macrophages by the alkali-extracted polysaccharide from spore of Ganoderma lucidum]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Mar;20(2):142-4.
[Article in Chinese]

Abstract

Aim: To investigate the activation of mouse macrophages by the alkali-extracted polysaccharides from the spore of Ganoderma lucidum (LZSBS).

Methods: The mouse macrophages cultured in-vitro were stimulated by LZSBS. IL-1beta and TNF-alpha in the culture supernatants were detected by ELISA. NO production was detected by Griess assay. The percentage of phagocytosis of latex beads by mouse macrophages was counted under microscope.

Results: The mouse macrophages stimulated by LZSBS increased in volume and darkened in appearance under phase-contrast microscope. LZSBS-activated mouse macrophages secreted IL-1beta and TNF-alpha produced a large amount of NO. The percentage of phagocytosis of latex beads by mouse macrophages was also significantly increased in the presence of LZSBS.

Conclusion: LZSBS can activate markedly the mouse macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Interleukin-1 / metabolism*
  • Macrophage Activation*
  • Macrophages / drug effects
  • Macrophages / metabolism*
  • Mice
  • Mice, Inbred BALB C
  • Nitric Oxide / metabolism
  • Phagocytosis / drug effects
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Reishi* / chemistry
  • Spores, Fungal / chemistry
  • Tumor Necrosis Factor-alpha / metabolism

Substances

  • Interleukin-1
  • Polysaccharides
  • Tumor Necrosis Factor-alpha
  • Nitric Oxide