[The effect of polyporus umbellatus polysaccharide on the immunosuppression property of culture supernatant of S180 cells]

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2004 Mar;20(2):234-7.
[Article in Chinese]


Aim: To investigate the effect of polyporus umbellatus polysaccharide (PUPS) on the immunosuppression property of tumor cell line S180 culture supernatant.

Methods: The inhibitory effects of the culture supernatant of S180 cells in the presence or absence of PUPS on the ConA-induced mouse splenocyte proliferation, IL-2 production, killer activity and the reactivity of CTLL-2 cells to IL-2 were detected by MTT colorimetry. The effect of the culture supernatant on the IL-2Ralpha expression on murine splenocytes was detected by flow cytometry.

Results: The culture supernatant of S180 cells could strongly inhibit immunity in terms of the above five indexes, while PUPS could reverse the immunosuppression.

Conclusion: PUPS can offset the immunosuppression of the supernatant from S180 cell culture, which may be mediated by down-regulating the synthesis and/or secretion of immunosuppressive substance by S180 cells.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Culture Media, Conditioned / pharmacology*
  • Female
  • Immunosuppressive Agents / pharmacology
  • Interleukin-2 / biosynthesis
  • Interleukin-2 Receptor alpha Subunit
  • Male
  • Mice
  • Polyporaceae* / chemistry
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Receptors, Interleukin-2 / biosynthesis
  • Sarcoma 180 / metabolism*
  • Sarcoma 180 / pathology
  • Spleen / cytology
  • Spleen / metabolism


  • Culture Media, Conditioned
  • Il2ra protein, mouse
  • Immunosuppressive Agents
  • Interleukin-2
  • Interleukin-2 Receptor alpha Subunit
  • Polysaccharides
  • Receptors, Interleukin-2