Expression analysis of endogenous retroviral elements belonging to the HERV-F family from human tissues and cancer cells

Joo-Mi Yi; Heui-Soo Kim

doi:10.1016/j.canlet.2004.01.026

Expression analysis of endogenous retroviral elements belonging to the HERV-F family from human tissues and cancer cells

Cancer Lett. 2004 Jul 28;211(1):89-96. doi: 10.1016/j.canlet.2004.01.026.

Authors

Joo-Mi Yi¹, Heui-Soo Kim

Affiliation

¹ Division of Biological Sciences, College of Natural Sciences, Pusan National University, Pusan 609-735, South Korea.

PMID: 15194220
DOI: 10.1016/j.canlet.2004.01.026

Abstract

Based on the primer binding site for Phe-tRNA, human endogenous retroviruses (HERVs) have been classified as HERV-F family members, HERV-F, HERV-FXA, HERV-Fb, and HERV-Fc. The HERV-F family was expressed in human placenta preferentially. We investigated pol fragments of HERV-F family in various human tissues and cancer cells. The pol gene was expressed in human placenta only among tissues (brain, prostate, testis, heart, kidney, liver, lung, placenta, skeletal muscle, spleen, thymus, uterus), but detected in various cancer cell lines (RT4, BT-474, HCT-116, TE-1, UO-31, Jurkat, A549, MCF7, OVCAR-3, MIA-PaCa-2, PC3, LOX-IMVI, AZ521, 2F7, U-937, and C-33A) except for PFSK-1 and HepG2 by RT-PCR analysis. Expression and sequencing data of the 37 clones imply that the pol gene of HERV-F family is active in cancer cells, which may have potential role related to cancer development.

MeSH terms

Amino Acid Sequence
Endogenous Retroviruses / genetics*
Genes, pol
Humans
Molecular Sequence Data
Neoplasms / genetics*
Phylogeny
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured