Critical functions for c-Myb at three checkpoints during thymocyte development

Nat Immunol. 2004 Jul;5(7):721-9. doi: 10.1038/ni1085. Epub 2004 Jun 13.


The transcription factor c-Myb is expressed throughout T cell development in the thymus. However, little is understood about c-Myb function because of the embryonic lethality of traditional Myb-null mutations. Using tissue-specific deletion to abrogate c-Myb expression at distinct stages of T cell development, we identify three points at which c-Myb activity is required for normal T cell differentiation: transition through the double-negative 3 stage, survival of preselection CD4(+)CD8(+) thymocytes, and differentiation of CD4 thymocytes. Thus, c-Myb is essential at several stages during T cell development in the thymus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • CD4-Positive T-Lymphocytes / cytology
  • CD4-Positive T-Lymphocytes / metabolism
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / metabolism
  • Cell Differentiation*
  • Cell Survival
  • Gene Deletion
  • Gene Expression Regulation
  • Gene Rearrangement, T-Lymphocyte / genetics
  • Mice
  • Mice, Transgenic
  • Proto-Oncogene Proteins c-myb / deficiency
  • Proto-Oncogene Proteins c-myb / genetics
  • Proto-Oncogene Proteins c-myb / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Recombination, Genetic / genetics
  • Thymus Gland / cytology*
  • Thymus Gland / metabolism*
  • Thymus Gland / pathology


  • Proto-Oncogene Proteins c-myb
  • RNA, Messenger