Differential distribution of TASK-1, TASK-2 and TASK-3 immunoreactivities in the rat and human cerebellum

Cell Mol Life Sci. 2004 Jun;61(12):1532-42. doi: 10.1007/s00018-004-4082-3.


In this work, the distributions of some acid-sensitive two-pore-domain K+ channels (TASK-1, TASK-2 and TASK-3) were investigated in the rat and human cerebellum. Astrocytes situated in rat cerebellar tissue sections were positive for TASK-2 channels. Purkinje cells were strongly stained and granule cells and astrocytes were moderately positive for TASK-3. Astrocytes isolated from the hippocampus, cerebellum and cochlear nucleus expressed TASK channels in a primary tissue culture. Our results suggest that TASK channel expression may be significant in the endoplasmic reticulum of the astrocytes. The human cerebellum showed weak TASK-2 immunolabelling. The pia mater, astrocytes, Purkinje and granule cells demonstrated strong TASK-1 and TASK-3 positivities. The TASK-3 labelling was stronger in general, but it was particularly intense in the Purkinje cells and pia mater.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Astrocytes / metabolism
  • Blotting, Western
  • Cells, Cultured
  • Cerebellum / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Endoplasmic Reticulum / metabolism
  • Hippocampus / metabolism
  • Humans
  • Immunohistochemistry
  • Microscopy, Fluorescence
  • Nerve Tissue Proteins / biosynthesis*
  • Pia Mater / metabolism
  • Potassium Channels / biosynthesis*
  • Potassium Channels, Tandem Pore Domain*
  • Purkinje Cells / metabolism
  • Rats
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transfection


  • KCNK5 protein, human
  • KCNK9 protein, human
  • Kcnk5 protein, rat
  • Kcnk9 protein, rat
  • Nerve Tissue Proteins
  • Potassium Channels
  • Potassium Channels, Tandem Pore Domain
  • potassium channel subfamily K member 3