Abstract
Background:
Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown.
Results:
We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction.
Conclusion:
These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzamides / pharmacology
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Blastocyst / chemistry
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Blastocyst / enzymology
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Blastocyst / metabolism*
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Chromatin Assembly and Disassembly / physiology
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Crosses, Genetic
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Embryo, Mammalian / chemistry
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Embryo, Mammalian / embryology
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Embryonic and Fetal Development / physiology*
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Epigenesis, Genetic / drug effects
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Epigenesis, Genetic / physiology
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Female
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Immunohistochemistry
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Inbred CBA
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Morula / drug effects
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Morula / enzymology
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Morula / metabolism
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Oocytes / chemistry
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Oocytes / enzymology
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Oocytes / growth & development
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Poly Adenosine Diphosphate Ribose / immunology
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Poly Adenosine Diphosphate Ribose / metabolism
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Poly Adenosine Diphosphate Ribose / physiology*
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Poly(ADP-ribose) Polymerase Inhibitors
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Poly(ADP-ribose) Polymerases / physiology
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Polymers / metabolism
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Zygote / enzymology
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Zygote / growth & development
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Zygote / physiology
Substances
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Benzamides
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Poly(ADP-ribose) Polymerase Inhibitors
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Polymers
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Poly Adenosine Diphosphate Ribose
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3-aminobenzamide
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Poly(ADP-ribose) Polymerases