Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development

BMC Mol Biol. 2004 Jun 16;5:4. doi: 10.1186/1471-2199-5-4.

Abstract

Background: Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown.

Results: We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction.

Conclusion: These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzamides / pharmacology
  • Blastocyst / chemistry
  • Blastocyst / enzymology
  • Blastocyst / metabolism*
  • Chromatin Assembly and Disassembly / physiology
  • Crosses, Genetic
  • Embryo, Mammalian / chemistry
  • Embryo, Mammalian / embryology
  • Embryonic and Fetal Development / physiology*
  • Epigenesis, Genetic / drug effects
  • Epigenesis, Genetic / physiology
  • Female
  • Immunohistochemistry
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred CBA
  • Morula / drug effects
  • Morula / enzymology
  • Morula / metabolism
  • Oocytes / chemistry
  • Oocytes / enzymology
  • Oocytes / growth & development
  • Poly Adenosine Diphosphate Ribose / immunology
  • Poly Adenosine Diphosphate Ribose / metabolism
  • Poly Adenosine Diphosphate Ribose / physiology*
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Poly(ADP-ribose) Polymerases / physiology
  • Polymers / metabolism
  • Zygote / enzymology
  • Zygote / growth & development
  • Zygote / physiology

Substances

  • Benzamides
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Polymers
  • Poly Adenosine Diphosphate Ribose
  • 3-aminobenzamide
  • Poly(ADP-ribose) Polymerases