Corneal wound healing in monkeys after repeated excimer laser photorefractive keratectomy

Arch Ophthalmol. 1992 Sep;110(9):1286-91. doi: 10.1001/archopht.1992.01080210104035.


Five rhesus monkey eyes underwent repeated argon fluoride (193 nm) excimer laser myopic photorefractive keratectomy 3 months following an initial ablation that had produced mild subepithelial haze. At 3 months all eyes had development of a dense subepithelial opacity and a thickened epithelium (12 cells, 80 microns) with vacuolization of basal cells, fragmented basement membrane, and a layer of subepithelial fibrosis containing activated fibroblasts. By 6 months the opacity was clearing; epithelium was thinner (50 microns); subepithelial fibrosis was more lamellar. By 15 months only mild haze persisted clinically; epithelium was 30 microns thick, with persistent basal vacuolization and focal basement membrane disruption; subepithelial fibrous tissue was more organized. Early repeated excimer laser ablation of the monkey cornea apparently induces vigorous stromal wound healing. Use of shallower ablations, corticosteroids, or a longer delay between ablations may be necessary for repeated laser surgery to be practical clinically.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Basement Membrane / pathology
  • Cornea / pathology
  • Cornea / physiopathology
  • Cornea / surgery*
  • Corneal Opacity / pathology
  • Corneal Stroma / pathology
  • Epithelium / pathology
  • Fibrosis
  • Laser Therapy*
  • Macaca mulatta
  • Myopia / pathology
  • Myopia / physiopathology
  • Myopia / surgery
  • Reoperation
  • Wound Healing*