Suppression of neutrophil recruitment in mice by geranium essential oil

Mediators Inflamm. 2004 Feb;13(1):21-4. doi: 10.1080/09629350410001664798.


Background: In aromatherapy, essential oils are used as anti-inflammatory remedies, but experimental studies on their action mechanisms are very limited.

Aims of the study: To assess their anti-inflammatory activities, the effects of essential oils on neutrophil recruitment in mice were examined in vivo.

Method: The effect of essential oils on leukocyte and neutrophil recruitment induced 6 h after intraperitoneal injection of casein in mice was examined.

Results: Leukocyte recruitment into the peritoneal cavity in mice was suppressed by intraperitoneal injections of geranium, lemongrass and spearmint oils at the dose of 5 microl/mouse, but was not by tea tree oil. This recruitment was inhibited dose-dependently by geranium oil. The suppression of leukocyte recruitment resulted from inhibition of neutrophil accumulation.

Conclusion: Some essential oils used as anti-inflammatory remedies suppress neutrophil recruitment into the peritoneal cavity in mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / administration & dosage
  • Anti-Inflammatory Agents / pharmacology*
  • Dose-Response Relationship, Drug
  • Female
  • Geranium / chemistry*
  • Injections, Intraperitoneal
  • Mentha spicata / chemistry
  • Mice
  • Mice, Inbred ICR
  • Neutrophil Infiltration / drug effects*
  • Oils, Volatile / administration & dosage
  • Oils, Volatile / pharmacology*
  • Peritoneal Cavity / cytology
  • Plant Oils / administration & dosage
  • Plant Oils / pharmacology*
  • Terpenes / administration & dosage
  • Terpenes / pharmacology


  • Anti-Inflammatory Agents
  • Oils, Volatile
  • Plant Oils
  • Terpenes
  • lemongrass oil