Effect of hyperoxia on interleukin-8 expression in premature versus term rabbit lung explants

Exp Lung Res. 2004 Jun;30(4):285-96. doi: 10.1080/01902140490276357.

Abstract

To examine whether prematurity significantly changes the lung inflammatory response to oxygen, rabbit lung explant cultures were exposed to 95% or 5% oxygen for 24 hours. Interleukin (IL)-8 protein concentrations from homogenates of the premature lung rose significantly after hyperoxia (6.8 +/- 1.8 in 5% O2 to 45.7 +/- 21.3 pg/microg protein in 95% O2) but not in the term lung (15.9 +/- 6.7 to 20.4 +/- 4.3 pg/microg protein). There was no change in IL-8 mRNA after hyperoxia in either age group. Preterm lungs demonstrated higher IL-8 levels by fluorescence-activated cell sorting (FACs) analysis and immunohistochemistry. This model may help determine why premature lungs are more susceptible to oxygen-induced disease.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Flow Cytometry
  • Gene Expression Regulation / drug effects*
  • Hyperoxia / genetics
  • Hyperoxia / physiopathology*
  • In Vitro Techniques
  • Interleukin-8 / analysis
  • Interleukin-8 / biosynthesis
  • Interleukin-8 / genetics*
  • Lung / drug effects
  • Lung / metabolism*
  • Oxygen / pharmacology*
  • Pregnancy
  • Premature Birth / genetics*
  • Premature Birth / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Rabbits
  • Term Birth / genetics*
  • Term Birth / metabolism

Substances

  • Interleukin-8
  • RNA, Messenger
  • Oxygen