Simian virus 40 infection down-regulates the expression of nitric oxide synthase in human mesothelial cells

Cancer Res. 2004 Jun 15;64(12):4082-4. doi: 10.1158/0008-5472.CAN-04-0486.

Abstract

The cytotoxic effects of asbestos are partly mediated by the production of free radicals, including nitric oxide (NO). SV40 has been suggested to synergize with asbestos in the pathogenesis of malignant mesothelioma. Crocidolite asbestos fibers induced in human mesothelial and malignant mesothelioma cells a significant increase of NO synthase activity and expression, which was absent in SV40-infected cells. Furthermore, SV40 infection prevented the NF kappa B activation elicited by crocidolite in both mesothelial and mesothelioma cells. These data suggest that SV40, by inhibiting the synthesis of NO, could favor the survival of transformed, potentially neoplastic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Asbestos, Crocidolite / adverse effects
  • Cells, Cultured
  • Down-Regulation
  • Epithelial Cells / drug effects
  • Epithelial Cells / enzymology*
  • Epithelial Cells / virology*
  • Humans
  • Mesothelioma / enzymology*
  • Mesothelioma / etiology
  • Mesothelioma / virology
  • NF-kappa B / antagonists & inhibitors
  • NF-kappa B / metabolism
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Nitric Oxide Synthase / biosynthesis*
  • Nitric Oxide Synthase Type II
  • Polyomavirus Infections / enzymology
  • Polyomavirus Infections / virology
  • Simian virus 40 / physiology*
  • Tumor Virus Infections / enzymology
  • Tumor Virus Infections / virology

Substances

  • NF-kappa B
  • Asbestos, Crocidolite
  • NOS2 protein, human
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type II