The importance of virus-associated host ICAM-1 in human immunodeficiency virus type 1 dissemination depends on the cellular context

FASEB J. 2004 Aug;18(11):1294-6. doi: 10.1096/fj.04-1755fje. Epub 2004 Jun 18.


The primary objective of this study was to define whether the nature of virion-bound host cell membrane proteins influenced the process of human immunodeficiency virus 1 (HIV-1) capture and transmission. We pulsed cells of monocytoid lineage (established and primary) and CD4-negative epithelial cells transiently expressing DC-SIGN or LFA-1 with isogenic HIV-1 particles either devoid or bearing host-derived ICAM-1 or ICAM-3 before incubation with an indicator cell line. To our surprise, the ICAM-1/LFA-1 association was a more efficient transmission factor than the combined gp120/DC-SIGN and ICAM-3/DC-SIGN interactions. The involvement of the association between virus-bound ICAM-1 and its natural ligand LFA-1 in virus binding and carriage was confirmed when using more physiological cellular targets, i.e., human lymphoid tissues cultured ex vivo. However, the contribution of virus-anchored host ICAM-1 to the process of retention and transmission of HIV-1 could not be confirmed when using primary human cells of macrophage/dendritic lineage as transmitter cells and autologous CD4+ T lymphocytes as targets. Altogether these data underscore the complexity of factors participating in virus-cell contact and efficient dissemination of HIV-1 to target cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / physiology
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / physiology
  • Cell Lineage
  • Cells, Cultured / virology
  • Dendritic Cells / virology
  • Epithelial Cells / virology
  • Genes, Reporter
  • HIV-1 / chemistry
  • HIV-1 / physiology*
  • Humans
  • Intercellular Adhesion Molecule-1 / physiology*
  • Lectins, C-Type / genetics
  • Lectins, C-Type / physiology
  • Leukocytes, Mononuclear / virology*
  • Ligands
  • Lymphocyte Function-Associated Antigen-1 / genetics
  • Lymphocyte Function-Associated Antigen-1 / physiology*
  • Lymphoid Tissue / cytology
  • Lymphoid Tissue / virology*
  • Macrophages / virology
  • Monocytes / virology
  • Organ Culture Techniques
  • Protein Binding
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / physiology
  • Recombinant Fusion Proteins / physiology
  • Transfection
  • Virion / chemistry


  • Antigens, CD
  • Cell Adhesion Molecules
  • DC-specific ICAM-3 grabbing nonintegrin
  • ICAM3 protein, human
  • Lectins, C-Type
  • Ligands
  • Lymphocyte Function-Associated Antigen-1
  • Receptors, Cell Surface
  • Recombinant Fusion Proteins
  • Intercellular Adhesion Molecule-1