IgE testing in capillary blood

Pediatr Allergy Immunol. 2004 Jun;15(3):230-3. doi: 10.1111/j.1399-3038.2004.00142.x.


Serologic IgE testing is generally performed using serum, obtained by venepuncture. We tested whether paper-absorbed and eluted capillary blood, obtained by a less invasive method (finger prick) could be used for allergy testing in young children. This was performed by comparative IgE testing, using paper-absorbed blood/serum and serum. Practical applicability of the procedure was tested by assaying paper-absorbed and eluted blood, obtained from 640 children with complaints of prolonged coughing, for IgE to airborne allergens. We found that IgE testing, using paper-absorbed/eluted material and serum yields virtually identical results (mean ratio for positive samples: 1.01, 95% confidence interval: 0.58-1.75). Blood spot testing revealed that sensitization to inhalant allergens is not uncommon in preschool children (13% positive radioallergosorbent test [RAST] tests), which means that this procedure is a useful method for assaying allergic sensitization in children.

Publication types

  • Comparative Study

MeSH terms

  • Blood Specimen Collection / methods
  • Capillaries*
  • Child, Preschool
  • Cough / blood
  • Cough / etiology
  • Cough / immunology*
  • Humans
  • Immunoglobulin E / blood*
  • Infant
  • Radioallergosorbent Test
  • Respiratory Hypersensitivity / blood
  • Respiratory Hypersensitivity / diagnosis


  • Immunoglobulin E