Efficacy of ozone on survival and permeability of oral microorganisms

Oral Microbiol Immunol. 2004 Aug;19(4):240-6. doi: 10.1111/j.1399-302X.2004.00146.x.


In the present study, we examined the effect of ozonated water on oral microorganisms and dental plaque. Almost no microorganisms were detected after being treated with ozonated water (4 mg/l) for 10 s. To estimate the ozonated water-treated Streptococcus mutans, bacterial cells were stained with LIVE/DEAD BacLight Bacterial Viability Kit. Fluorescence microscopic analysis revealed that S. mutans cells were killed instantaneously in ozonated water. Some breakage of ozonated water-treated S. mutans was found by electron microscopy. When the experimental dental plaque was exposed to ozonated water, the number of viable S. mutans remarkably decreased. Ozonated water strongly inhibited the accumulation of experimental dental plaque in vitro. After the dental plaque samples from human subjects were exposed to ozonated water in vitro, almost no viable bacterial cells were detected. These results suggest that ozonated water should be useful in reducing the infections caused by oral microorganisms in dental plaque.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aggregatibacter actinomycetemcomitans / drug effects*
  • Biofilms / drug effects
  • Cell Membrane Permeability / drug effects
  • Chlorhexidine / analogs & derivatives*
  • Chlorhexidine / pharmacology
  • Dental Plaque / drug therapy
  • Dental Plaque / microbiology
  • Disinfectants / therapeutic use
  • Humans
  • Mouth / microbiology
  • Ozone / pharmacology*
  • Ozone / therapeutic use
  • Porphyromonas / drug effects*
  • Povidone-Iodine / pharmacology
  • Sterilization
  • Streptococcus mutans / drug effects*


  • Disinfectants
  • Ozone
  • Povidone-Iodine
  • chlorhexidine gluconate
  • Chlorhexidine