Anti-glutamic acid decarboxylase antibodies in the serum and cerebrospinal fluid of patients with stiff-person syndrome: correlation with clinical severity

Arch Neurol. 2004 Jun;61(6):902-4. doi: 10.1001/archneur.61.6.902.

Abstract

Background: Stiff-person syndrome (SPS) is an immune-mediated central nervous system disorder characterized by fluctuating muscle stiffness, disabling spasms, and heightened sensitivity to external stimuli. Up to 80% of patients with SPS have anti-glutamic acid decarboxylase (GAD) antibodies in the serum or cerebral spinal fluid (CSF). Whether these antibodies are clinically relevant and correlate with disease severity is unknown.

Objective: To correlate anti-GAD antibody titers in the serum and CSF of patients with SPS with the degree of clinical severity.

Design: Patients studied the last 6 years.

Setting: The Clinical Center of the National Institutes of Health, Bethesda, MD.

Patients: Sixteen patients with typical SPS and elevated serum anti-GAD antibody titers.

Interventions: Antibody titers in serum and CSF were measured by radioimmunoassay, and the intrathecal anti-GAD-specific IgG production was calculated.

Main outcome measures: Comparison of antibody titers with stiffness index and heightened sensitivity scores based on scales that reliably measure disease severity.

Results: The mean disease duration was 11 years (range, 5-30 years). The mean anti-GAD antibody titer in the serum was 51 500 U/mL (range, 24 000-200 000 U/mL); and in the CSF, 181 U/mL (range, 30-400 U/mL). A 10-fold increased intrathecal production of GAD-specific IgG antibodies was noted. No correlation was found between antibody titers in serum or CSF with disease severity. In 4 patients, the anti-GAD antibody titers measured serially during a 2-year period did not correlate with clinical fluctuations.

Conclusions: In patients with SPS, the anti-GAD antibody titers in serum and CSF do not correlate with disease severity or duration. Anti-GAD antibodies are an excellent marker for SPS, but monitoring their titers during the course of the disease may not be of practical value.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Autoantibodies / biosynthesis
  • Autoantibodies / blood*
  • Autoantibodies / cerebrospinal fluid*
  • Autoantigens / immunology
  • Biomarkers / blood
  • Biomarkers / cerebrospinal fluid
  • Female
  • Glutamate Decarboxylase / immunology*
  • Humans
  • Male
  • Middle Aged
  • Severity of Illness Index
  • Stiff-Person Syndrome / enzymology*
  • Stiff-Person Syndrome / immunology

Substances

  • Autoantibodies
  • Autoantigens
  • Biomarkers
  • Glutamate Decarboxylase