Heat Shock and Cold Shock in Deinococcus Radiodurans

Cell Biochem Biophys. 2004;40(3):277-88. doi: 10.1385/CBB:40:3:277.

Abstract

On the basis of acquired thermotolerance and cryotolerance, the optimal heat shock and cold shock temperatures have been determined for Deinococcus radiodurans. A heat shock at 42 degrees C maximized survival at the lethal temperature of 52 degrees C and a cold shock at 20 degrees C maximized survival after repeated freeze-thawing. Enhanced survival from heat shock was found to be strongly dependent on growth stage, with its greatest effect shortly after phase. Increased synthesis of a total of 67 proteins during heat shock and 42 proteins during cold shock were observed by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and autoradiography. Eight of the most highly induced heat shock proteins shown by 2D PAGE were identified by MALDI-MS as Hsp20, GroEL, DnaK, SodA, Csp, Protease I, and two proteins of unknown function.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Autoradiography
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism
  • Cold Temperature
  • Deinococcus / metabolism*
  • Electrophoresis, Gel, Two-Dimensional
  • Heat-Shock Proteins / isolation & purification
  • Heat-Shock Proteins / metabolism
  • Heat-Shock Response
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Bacterial Proteins
  • Heat-Shock Proteins