Subcellular localization of EEN/endophilin A2, a fusion partner gene in leukaemia

Biochem J. 2004 Oct 1;383(Pt 1):27-35. doi: 10.1042/BJ20040041.


EEN (extra eleven nineteen), also known as EA2 (endophilin A2), a fusion partner of the MLL (mixed-lineage leukaemia) gene in human acute leukaemia, is a member of the endophilin A family, involved in the formation of endocytic vesicles. We present evidence to show that EEN/EA2 is localized predominantly in nuclei of various cell lines of haemopoietic, fibroblast and epithelial origin, in contrast with its reported cytoplasmic localization in neurons and osteoclasts, and that EEN/EA2 exhibits nucleocytoplasmic shuttling. During the cell cycle, EEN/EA2 shows dynamic localization: it is perichromosomal in prometaphase, co-localizes with the bipolar spindle in metaphase and anaphase and redistributes to the midzone and midbody in telophase. This pattern of distribution coincides with changes in protein levels of EEN/EA2, with the highest levels being observed in G2/M-phase. Our results suggest that distinct subcellular localization of the endophilin A family members probably underpins their diverse cellular functions and indicates a role for EEN/EA2 in the cell cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Specificity
  • Cell Cycle / physiology*
  • Cell Line
  • Cell Line, Tumor
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • Endocytosis / physiology*
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Leukemia / metabolism
  • Mitosis / physiology
  • Protein Transport
  • Proteins / immunology
  • Proteins / metabolism
  • Proteins / physiology*
  • Sequence Deletion
  • Subcellular Fractions / metabolism*
  • src Homology Domains


  • Intracellular Signaling Peptides and Proteins
  • Proteins
  • SH3GL1 protein, human